The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways

Abstract Background Human adipose-derived stem cells (hADSCs) are with the potential to differentiate in multiple directions. miR-204-5p is expressed at low levels during osteogenic differentiation of hADSCs, and its specific regulatory mechanism remains unclear. Here, we aimed explore function possible molecular hADSCs. Methods The expression patterns miR-204-5p, Runx2 , alkaline phosphatase ( ALP ), osteocalcin OCN forkhead box C1 FOXC1 ) growth factor 7 GDF7 hADSCs osteogenesis were detected by qRT-PCR. Then, alizarin red staining (ARS) used detect osteoblast activities mineral deposition. Western blotting was conducted confirm protein levels. relationship among verified dual-luciferase activity chromatin immunoprecipitation (ChIP) assays. Results downregulated hADSC osteogenesis, overexpression suppressed differentiation. Furthermore, decreased mimics group, which indicates that suppresses binding their 3′-untranslated regions (UTRs). Overexpression or improved inhibition induced mimics. Moreover, found bind promoter GDF7, promote deacetylation reduce thus promoting activating AKT P38 signalling pathways. Conclusions Our results demonstrated miR-204-5p/FOXC1/GDF7 axis regulates via p38 This study further revealed from perspective miRNA regulation.